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macro prep r ceramic hydroxyapatite type 1  (Bio-Rad)


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    Bio-Rad macro prep r ceramic hydroxyapatite type 1
    Macro Prep R Ceramic Hydroxyapatite Type 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macro+prep+r+ceramic+hydroxyapatite+type+1/CHT+Ceramic+Hydroxyapatite/us08952137-329-5-11
    Average 96 stars, based on 1341 article reviews
    macro prep r ceramic hydroxyapatite type 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Purification:

    Article Title: Method for producing high-purity soluble thrombomodulin
    Article Snippet: .. 20 ml out of the purified product obtained in Example 10 was subjected to a Macro-Prep (R) Ceramic Hydroxyapatite TYPE 1 (BIO-RAD) column (diameter: 0.5 cm; height: 9.7 cm) that had been equilibrated with a 20 mM HEPES buffer solution (pH 7) containing 0.17M sodium chloride and 2 mM sodium phosphate. ..

    Article Title: Method for producing high-purity soluble thrombomodulin
    Article Snippet: A specific example is a purification method comprising: roughly purifying a culture supernatant with Q-Sepharose FF used as an ion-exchange column to recover a fraction having thrombomodulin activity; purifying the recovered fraction with a mouse anti-thrombomodulin monoclonal antibody on an affinity column to recover a fraction having high thrombomodulin activity; purifying the recovered fraction with SP-Sepharose FF used as a cation-exchange column; concentrating a pass-through fraction; and then subjecting the resultant fraction to gel filtration to obtain a thrombomodulin active fraction. .. After completion of such purification with SP-Sepharose FF, the anion exchanger of the present invention, preferably an anion exchanger having a quaternary ammonium group such as SourceQ (GE Healthcare Bio-Sciences), which is a strong anion exchanger, or the hydroxyapatite of the present invention such as Macro-Prep (R) Ceramic Hydroxyapatite TYPE 1 (BIO-RAD), is used under optimal conditions, so as to easily obtain high-purity thrombomodulin that does not substantially contain a denatured product of soluble thrombomodulin. .. On the other hand, after purification with a mouse anti-thrombomodulin monoclonal antibody, the anion exchanger of the present invention, preferably an anion exchanger having a quaternary ammonium group such as SourceQ (GE Healthcare Bio-Sciences), which is a strong anion exchanger, is used under optimal conditions, so that a denatured product of soluble thrombomodulin, a mouse antibody-derived product, and in the case of using a serum-containing medium, a serum-derived product can be simultaneously eliminated, thereby easily obtaining high-purity thrombomodulin that does not substantially contain a denatured product of soluble thrombomodulin.



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    macro prep r ceramic hydroxyapatite type 1  (Bio-Rad)
    96
    Bio-Rad macro prep r ceramic hydroxyapatite type 1
    Macro Prep R Ceramic Hydroxyapatite Type 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macro+prep+r+ceramic+hydroxyapatite+type+1/CHT+Ceramic+Hydroxyapatite/us08952137-329-5-11
    Average 96 stars, based on 1 article reviews
    macro prep r ceramic hydroxyapatite type 1 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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